Antonio Adamo

Principal Investigator

Associate Professor, Biological and Environmental Sciences and Engineering Division (BESE)

Current

Location:

Bldg. 2, level 3, Room 3337

Social Profile:

Research Interests

​Professor Adamo’s research interests focus on use of human embryonic stem cells (hESCs) and patient-derived induced pluripotent stem cells (iPSCs) to model the onset and progression of human disorders linked to copy number variations “in a dish.” My team developed the largest cohort of Klinefelter syndrome (karyotype 47,XXY) and high-grade X aneuploid iPSCs (karyotype 48,XXXY and 49,XXXXY) that we use to study the molecular dysregulations associated with X chromosome aneuploidy during the earliest stages of human development. We combine reprogramming, organoid derivation, and genome editing techniques with a multi-omics approach to identify the transcriptional and epigenetic signatures underlying human diseases.

Selected Publications

Astro V, Ramirez-Calderon G, Adamo A.STAR Protoc. 2023 Apr 13;4(2):102252. doi: 10.1016/j.xpro.2023.102252. Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media. Online ahead of print. PMID: 37060558. 

Astro V, Fiacco E, Cardona Londoño KJ, De Toma I, Alzahrani HS, Alama J, Kokandi A, Hamoda TAA, Felemban M, Adamo A.Endocr Connect. 2023 Mar 1:EC-22-0515. doi: 10.1530/EC-22-0515. A transcriptomic signature of X chromosome overdosage in Saudi Klinefelter syndrome induced pluripotent stem cells.Online ahead of print. PMID: 36971776

Astro V, Ramirez-Calderon G, Pennucci R, Caroli J, Saera-Vila A, Cardona-Londoño KJ, Forastieri C, Fiacco E, Ramirez-Calderon G, Maksoud F, Alowaysi M, Sogne E, Falqui A, Gonzàlez F, Montserrat N, Battaglioli E, Mattevi A, Adamo A*. Fine-tuned KDM1A alternative splicing regulates human cardiomyogenesis through an enzymaticindependent mechanism iScience. 2022 Jun 23;25(7):104665. doi: 10.1016/j.isci.2022.104665 *Corresponding author

Astro V†, Al-Owaysi M†, Fiacco E†, Saera-Vila A, Cardona- Londoño KJ, Cigliano RA, Adamo A*. Pseudoautosomal Region 1 Overdosage Affects the Global Transcriptome in iPSCs From Patients With Klinefelter Syndrome and High-Grade X Chromosome Aneuploidies. Front. Cell Dev. Biol., 03 February 2022 https://doi.org/10.3389/fcell.2021.801597. PMID: 35186953. †equal contribution, *Corresponding author

Ramirez-Calderon G†, Colombo G†, Hernandez-Bautista CA, Astro V, Adamo A*: Heart in a Dish: From Traditional 2D Differentiation Protocols to Cardiac Organoids. Frontiers in Cell and Developmental Biology eCollection 2022. †equal contribution, *Corresponding author

Alowaysi M, Astro V, Fiacco E, Alzahrani F, Alkuraya, FS, Adamo A*: Generation of iPSC lines (KAUSTi011-A, KAUSTi011-B) from a Saudi patient with epileptic encephalopathy carrying homozygous mutation in the GLP1R gene. Stem Cell Research Volume 50, 2021 January. *Corresponding author

Fiacco E, Alowaysi M, Astro V, Adamo A*: Derivation of two naturally isogenic iPSC lines (KAUSTi006-A and KAUSTi006-B) from a mosaic Klinefelter Syndrome patient (47-XXY/46-XY). Stem Cell Research Volume 49 December 2020. *Corresponding author

Alowaysi M, Fiacco E, Astro V, Adamo A*: Establishment of an iPSC cohort from three unrelated 47-XXY Klinefelter Syndrome patients (KAUSTi007-A, KAUSTi007-B, KAUSTi009-A, KAUSTi009-B, KAUSTi010-A, KAUSTi010-B). Stem Cell Research Volume 49 December 2020. *Corresponding author

Alowaysi M, Fiacco E, Astro V, Adamo A*: Establishment of iPSC lines from a high-grade Klinefelter Syndrome patient (49-XXXXY) and two genetically matched healthy relatives (KAUSTi003-A, KAUSTi004-A, KAUSTi004-B, KAUSTi005-A, KAUSTi005-B, KAUSTi005-C). Stem Cell Research Volume 49 December 2020. *Corresponding author

Fiacco E, Alowaysi M, Astro V, Adamo A*: Generation of an iPSC cohort of isogenic iPSC lines (46-XY and 47- XXY) from a non-mosaic Klinefelter Syndrome Patient (47-XXY) (KAUSTi008-A, KAUSTi008-B, KAUSTi008-C, KAUSTi008-D, KAUSTi008-E, KAUSTi008-F, KAUSTi008-G). Stem Cell Research Volume 50 2021 January. *Corresponding author

Alowaysi M, Astro V, Fiacco E, Adamo A*: Generation of two iPSC lines (KAUSTi001-A, KAUSTi002-A) from a rare high-grade Klinefelter Syndrome patient (49-XXXXY) carrying a balanced translocation t(4,11) (q35,q23). Stem Cell Research Volume 49 December 2020. *Corresponding author

Alam T, Alazmi M, Naser R, Huser F, Momin AA, Astro V, Hong S, Walkiewicz KW, Canlas CG, Huser R, Ali AJ, Merzaban J, Adamo A, Jaremko M, Jaremko Ł, Bajic VB, Gao X, Arold ST. (2019) Proteome-level assessment of origin, prevalence and function of Leucine-Aspartic Acid (LD) motifs. Bioinformatics. 2019 Oct 4.

Astro V. Adamo A.* (2018) Epigenetic Control of Endocrine Pancreas Differentiation in vitro: Current Knowledge and Future Perspectives. Frontiers in Cell and Dev. Bio. 2018; 6: 141. * Corresponding author

Germain PL, Vitriolo A, Adamo A, Laise P, Das V, Testa G (2016) RNAontheBENCH: computational and empirical resources for benchmarking RNAseq quantification and differential expression methods. Nucleic Acids Res. 2016 Jun 20;44(11):5054-67

Education

  • Ph.D. in Biotechnologies applied to Medical Sciences, University of Milan, Milan (Italy), 2008
  • M.Sc., Medical Biotechnologies and Molecular Medicine, University of Milan, Milan (Italy), 2005
  • B.Sc., Medical Biotechnologies, University of Milan,  Milan (Italy), 2003

Professional Profile

- FEBS Senior Postdoctoral Fellow, European Institute of Oncology (IEO), Milan (Italy), 2012-2015

- Postdoctoral Fellow, Center of Regenerative Medicine of Barcelona (CMRB), Barcelona (Spain), 2009-2012

KAUST Affiliations

Biological and Environmental Science and Engineering Division (BESE)

Bioengineering (BioE)

KAUST Environmental Epigenetics Program (KEEP)

KAUST Smart Health Initiative (KSHI)